关键词:
纳米技术
纳料材料
精密工程
研究
摘要:
A portable instrument was designed for rapid field specific test of Escherichia coli (E. coli) based on bioluminescent magnetic immunoassay, which consisted of photon counting unit, central processing unit, temperature control unit and power supply unit. The temperature control unit supplied a constant temperature of 37 ℃, which is suitable for incubation and convenient for field test. An improved simplified coupling method was presented, in which immunomagnetic microbeads coupled with biotinylated rabbit-anti-E. coli antibody could capture E. coli directly by one step. The detecting result of E. coli showed a good linear relationship in lg-lg domain between the intensity of luminescence and the concentration of E. coli obtained through plate counting in the range from 23.00 CFU/mL to 2.25x 10~7 CFU/mL, with a correlation coefficient of 0.965 7. The sensitivity of the detecting system was 0.478 7. In the reproducibility test, the coefficient of variation was 4.48%. The instrument integrated with constant temperature control is portable, easy to operate and low in cost, which will provide potential applications in food hygiene and safety, environmental monitoring and disease diagnosis.